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Introducing New Generation RNA-SEQ Library Prep

All New

  • Fundamentally New Technology

  • Capture All RNA Biotypes in a Single Library Prep

  • Simple Workflow

All at High Sensitivity - Exceptional Performance with Picogram Inputs

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Fundamentally NEW Platform Based on 2Dzyme: Designed and Engineered for Next-Generation Library Prep

“The electric light did not come from continuous improvement of candles.”

- Orem Harari


For the first time…

Convert miRNA to Large RNA  in Single Library Prep.

Sequencing data from our prep show over 1300 unique miRNA along with over 19,000 unique protein coding transcripts using 10ng rRNA depleted Universal Human Reference RNA. 2D Complete RNA kit converts all RNA Biotypes in the same library prep. Libraries are prepared using the 2D Complete RNA Kit and sequenced with 1x100bp reads on the Illumina Platform. 

2D Complete RNA 


Simple Workflow

LOVED BY HUMANS. LOVED BY ROBOTS.

“With this protocol, I would love to be on the bench again!”

                              - Vice President (early access client)


Exceptional Performance even with Picogram Inputs

The data is from libraries prepared using the 2D Complete RNA kit with various input amounts of Human Brain mRNA. Data shows very consistent performance across 3 orders of magnitude of input range. Insets indicates Pearson coefficient of correlation. 


An inherently Stranded Approach - Greater than 99% Strand Accuracy

2D Genomics Library Prep kits retains > 99% strand accuracy. With 10ng Human Brain mRNA input, the library was prepared using 2D Complete RNA Kit. The data shows >99% reads correctly mapped to the originating strand of genomic DNA for PHC1 and M6PR transcripts.


Even Coverage Across High GC Content and Low Abundance Transcripts

Uniform coverage obtained with a broad range of RNA concentrations. The traces represent coverage of the FTL transcript; rich in GC content. The transcript is difficult to cover using popular commercial kits. Each green trace represents a different amount of sample inputs, from the top 0.1ng, 1ng, 10ng and 100ng of Human Brain mRNA. The bottom trace (blue) was generated with 10ng of UHR RNA, which was Ribo-depleted.

Improved detection and uniform coverage obtained with broad range of RNA concentrations of Low- abundance transcript MALAT1. This transcript is difficult to cover using other commercial kits.

The green traces represent different amount of sample inputs, from the top 0.1ng, 1ng, 10ng and 100ng of Human Brain mRNA. The bottom trace (blue) was generated with 10ng of UHR RNA which was Ribo-depleted.


 

optimized for liquid biopsy application using cell-free rna

Using 2D Complete RNA kit, library was prepared for cell-free RNA (cfRNA) and sequenced on Illumina Platform. The cfRNA was extracted from 5mL of Blood using Zymo Quick-cfRNA Serum & Plasma kit. The data shows conversion of All biotypes of RNA from sample type where the RNA is naturally degraded. 


Meet the 2D product lineup